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Biomedical Techniques (Chinese)

 

Biomedical Protocols

Preparation of Basic Reagents

DNA Extraction from Tissue/Cells

Plasmid DNA Isolation

RNA Extraction from Tissue Cells

Southern Blot Analysis

Northern Blot Analysis

Preparation of Acrylamide Gel (Bio-Rad)

Western Blotting Protocol

蛋白质免疫印迹(Western blot)中常见问题与解决方法

Stripping and Reusing Western Blots

sgRNA online design sites

SiRNA Transfection

Plasmid Transfection

Immunoprecipitation Protocol

Coimmunoprecipitation

Soft Agar Assay for Colony Formation

Paraffin embedding and sectioning

H&E staining of paraffin Slides

Matrigel Invasion Assay

FISH Protocol

Bisulfite Treatment of DNA

Immunohistochemistry Protocol (Paraffin)

Immunohistochemistry Protocol (Frozen)

Immunofluorescence Protocol

General PCR Protocol

Mouse Tail DNA Extraction Protocol

Vhl knockout mouse genotyping protocol

BHD Knockout Mouse Genotyping Protocol

Cre transgenic mouse genotyping protocol

Sglt2-Cre Transgenic Mouse Genotyping Protocol

Transwell Migration & Invasion

Biotin-Streptavidin Techology

Streptavidin Magnetic Beads protocol

ER-Cre transgenic mouse genotyping protocol

Ksp-Cre transgenic mouse genotyping protocol

FLPer transgenic mouse genotyping protocol

Neo (neomycin-resistant gene) transgenic mouse genotyping protocol

LacZ transgenic mouse genotyping protocol

LacZ staining protocol

Preparation of Copy Standards

BAF180 mouse genotyping protocol (MGH strain)

BAF250A mouse genotyping protocol (MGH strain)

Identification of UOK257 and UOK257-2 protocol

Isolation and Purification of Exosomes

Culture of human normal kidney and primary tumor tissues

Mouse mammary fat pad transplantation protocol

 

慢病毒包装手册

Animal Use and Care courses

Animal Protocols

Antibody

BioStatitics

Animal Models and Vivarium Setup

CTC-CTM-CtDNA

Liquid Biospy

Clinical-Trials

实验动物用药剂量换算汇总

CAR-T-PD-L1

Gene-Editing

Stem Cells

附件:氨基酸结构与性质

DNA-SequenceEditingComparingTools

Design and synthesis of labeled small molecular probes for identification of protein targets

Drug design and druggability

必需氨基酸

碱基核苷核苷酸

化学基团官能团汇总

杂环化合物汇总

药效团与分子对接相关软件

卤素及磺酰基团在药物分子中的作用与实例

先导化合物结构优化策略

改变代谢途径提高代谢稳定性

结构修饰降低潜在毒性

化学修饰改善水溶性

甲氧基在药物改构中的疏水作用

三个重要理化参数和计算预测

药物的吸收代谢和分布

计算机辅助药物设计导论

新药合成艺术

新药化学全合成路线手册

药物合成反应基础

肿瘤治疗的各类靶向药物

全球抗癌药和不限癌种的抗癌药汇总

激酶抑制剂汇总-CDK2 抑制剂

CDK 抑制剂药物的改构上市回顾

医药研发术语简写大全

小分子药物靶点鉴定的方法汇总

创新药上市申请流程

免疫治疗类别

Breeding of nude mice

胚胎冷冻和移植技术

小鼠尾静脉注射方法

冠状病毒类型结构与治疗药物

专利类别

PCT专利申请流程

经络

足少阴肾经

足太阳膀胱经

足少阳胆经

足阳明胃经

足太阴脾经

足厥阴肝经

手少阳三焦经

手太阳小肠经

手阳明大肠经

手太阴肺经

手少阴心经

手厥阴心包经

督脉穴位

任脉24个高清穴道图

冲脉

奇经八脉之带脉

阳维脉

阴维脉

阳跷脉

阴跷脉

十二经别、经筋、皮部

别络-简介

浮络和孙络

超高清版人体穴位经络大图【最全珍藏版】修正版

人体重要穴位与功用