PCR Primer Designing Websites
NCBI Primer-Blast
Primer3 Input
IDT OligoAnalyzer
IDT PrimerQuest (RT-PCR)
IDT Antisense Design
IDT RNAi Primer Design
GeneFisher-Interactive PCR Primer Design
Primer3
Primer3Plus
OligoCalc
RAPD-primer generator
NetPrimer
PathoGene
PrimerX (site-directed mutagenesis)
SiteFind (site-directed mutagenesis)
UCSC In-Silico PCR (search sequence by primer)
Web Primer
Primo Pro
QuantPrime (qRT-PCR)
BiosearchTech Probe-Primer Design
GenScript Real-time PCR (TaqMan) Primer Design
AutoPrimer (Real-time PCR)
ExonPrimer (Intronic primers)
PrimerBank (existing primer database)
Degenerate primers based on Protein sequence:
CODEHOP
PCR primers based upon multialignments:
Primaclade
PriFi
Genomic scale primers:
The PCR Suite
MuPlex
Introduction of silent mutations:
WatCut
Others:
PCR Box Titration Calculator
Sequence Extractor
Tm Calculator-dnaMate
Melting Calculator
|
